In situ hybridisation of CRH mRNA in the paraventricular nucleus of a rat.
(a) In situ hybridisation of CRH mRNA in the paraventricular nucleus of a normal rat. (b) Hybridisation of CRH mRNA in a rat following the intracerebroventricular administration of recombinant human IL-lb (100ng, followed by an infusion of 20ng/h for 12 hours). When compared with (a), there is readily detectable activation of CRH neuronal activity. By courtesy of Drs I Kakuschka and R Lechan.
